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The role of temperature in Solid Phase Peptide Synthesis
In this case study, we evaluate the effect of temperature on the speed of synthesis and crude purity.
Optimizing SPPS with Conduction Preheating
In a recent collaborative study with Beatriz G. de la Torre and Fernando Albercio at the University of KwaZulu-Natal, we explored the impact of thermal management on peptide quality. The study, published in TKS | TeknoScienze under the title "Conduction heating-assisted solid-phase peptide synthesis", showcases how conduction preheating of reagents prior to delivery to the jacketed reaction vessel drastically improves synthesis quality while significantly reducing cycle times.
The Power of Precision: Conduction vs. Microwave
While microwave-assisted synthesis is a common industry tool, it often lacks the thermal agility required for complex sequences. We utilized an in-line heat exchanger to provide tightly regulated temperature control throughout the entire synthesis process. Unlike other heating methods, conduction allows for both rapid heating and cooling. This thermal nimbleness ensures that peptides are not subjected to unnecessary heat stress, potentially reducing side reactions and degradation.
Every peptide is unique. Factors such as chain length, sequence composition, and synthesis scale dictate different optimal conditions. Our technology allows for precise, step-by-step temperature adjustments, ensuring each coupling and deprotection cycle is optimized for maximum yield and purity. In this study, we successfully synthesized challenging model peptides, demonstrating that preheating solvents and reagents ensures a more homogeneous reaction environment from the moment they contact the resin.
Future-Proofing with Green Chemistry
Precise thermal regulation is also a cornerstone of the industry's shift toward sustainability. As hazardous solvents like DMF and NMP are phased out, green solvents are becoming the new standard. Many of these alternatives require specific, tightly controlled thermal profiles to maintain optimal viscosity and reaction conditions. Our conduction heating and cooling technology is designed to meet these evolving requirements, ensuring your lab stays at the forefront of both science and environmental responsibility.
This article was originally published in TKS | TeknoScienze, Vol. 43(6), 2025. "Conduction heating-assisted solid-phase peptide synthesis." The original publication and full author list can be found here.
Download the publication here
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CSBio is a leading peptide instrumentation manufacturing company located in Silicon Valley, California.
CSBio provides research scale peptide synthesizers, pilot scale peptide synthesizers, commerical scale peptide synthesizers, and DNA/RNA oligonucleotide synthesizers.