A Mini Course on Solid Phase Peptide Synthesis (SPPS)
In this series, we provide a 2 hour mini-course on the fundamentals and how to perform solid phase peptide syntehsis
Introduction
At CSBio, we regularly work with labs and universities that have never made a peptide before. In the process of providing them with a peptide synthesizer, we give them hands on training to quickly allow them to start making peptides.
In this mini-course, we provide an overview on what peptides are and how to synthesize peptides.
Course Introduction
An Introduction
A brief overview and introduction to your instructor for this mini-course.
The fundamentals
Amimo Acids
What are amino acids?
Proteins and Peptides
What are proteins and peptides?
Solid Phase Peptide Synthesis
How to make a peptide
How a peptide is made: solution phase and solid phase peptide synthesis
Different SPPS activation chemistries
Discussion on the activation chemistry and making the peptide bond. We discuss the most common chemistries DIC and HBTU.
Resin Types and Applications
Discussing the "solid phase" part of SPPS - common resin types in SPPS and when certain resins are used.
Units in SPPS
Discussing the units used in SPPS
An example
An example of how to make a peptide with SPPS
Preparation of Reagents
How to prepare reagents when making a peptide with SPPS
The Synthesis Cycle
Discussing the synthesis cycle in SPPS
SPPS in the Lab
Making a Peptide Manually
Walking through how a peptide is synthesized manually in the lab.
Making a Peptide Automated
Walking through how a peptide is synthesized with an automated peptide synthesizer in the lab.
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About Us
CSBio is a leading peptide instrumentation manufacturing company located in Silicon Valley, California.
CSBio provides research scale peptide synthesizers, pilot scale peptide synthesizers, commerical scale peptide synthesizers, and DNA/RNA oligonucleotide synthesizers.