Peptide Analysis

HPLC analysis RP-HPLC (reversed phase) SEC-HPLC (size exclusion) IE-HPLC (ion exchange) method develpment and validation for Non-GMP and GMP projects. Mass Spectrometric analysis ESI-MS full scan LC-MS LC-MS-MS Amino Acid Analysis Proteins/peptides are hydrolyzed using HCl. The released amino acid residues are analyzed on a reversed phase HPLC after pre-column derivatization. Water Content Moisture Analysis by Karl Fisher titration Peptide content by elemental analysis Peptide counter ion content TFA, Acetic Acid content by HPLC Residual Solvent content DMF, DCM, ACN content by GC UV qualitative and quantitative analysis Melting Point Elemental Analysis C%, H%, and N% of organic compounds

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